Composite

Part:BBa_K1615101:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-31)


CBDcenA+Linker fused to mRFP driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.


Source

mRFP highly engineered variant originally from Discosoma striata. CBDcenA from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington.

References